Ethanol Precipitation of DNA and RNA How it works.Ethanol precipitation is a commonly used technique for concentrating and de salting nucleic acids DNA or RNA preparations in aqueous solution.The basic procedure is that salt and ethanol are added to the aqueous solution, which forces the precipitation of nucleic acid nucleic acids out of solution.After precipitation the nucleic acids can then be separated from the rest of the solution by centrifugation.The pellet is washed in cold 7.So how does this work Ethanol precipitation of nucleic acids is all about solubility First we need to know why nucleic acids are soluble in water.Bioland Scientific Transfection Reagent Buffers Chemicals Gloves Transfection Reagent Plasmid DNA Purification Virus Purification Kits Genomic DNA purification.I get many people complaining to me about poor DNA yields from commercial plasmid plasmid prep kits.In this article I will explain the main pitfalls in plasmid.Qiagen Plasmid Miniprep Kit Manuals RestaurantWater is a polar molecule it has a partial negative charge near the oxygen atom due the unshared pairs of electrons, and partial positive charges near the hydrogen atoms see the diagram on the right.Because of these charges, polar molecules, like DNA or RNA, can interact electrostatically with the water molecules, allowing them to easily dissolve in water.Polar molecules can therefore be described as hydrophilic and non polar molecules, which cant easily interact with water molecules, are hydrophobic.Nucleic acids are hydrophilic due to the negatively charged phosphate PO3 groups along the sugar phosphate backbone.The role of the salt in ethanol precipitationOk, so back to the protocol.The role of the salt in the protocol is to neutralize the charges on the sugar phosphate backbone.A commonly used salt is sodium acetate.Bioland Scientific Replacement Cartridge for QGARD00D2 RR400Q201 Product Description This product is designed to remove ionic and organic contaminates down to.For sensitive and reproducible PCR and RTPCR.NTP Set and dNTP Mix, PCR Grade are suitable for use in all standard PCR techniques and all sensitive PCR techniques.In solution, sodium acetate breaks up into Na and CH3.COO. The positively charged sodium ions neutralize the negative charge on the PO3 groups on the nucleic acids, making the molecule far less hydrophilic, and therefore much less soluble in water.The role of the ethanolThe electrostatic attraction between the Na ions in solution and the PO3 ions are dictated by Coulombs Law, which is affected by the dielectric constant of the solution. Bluetooth Hacking Software Bt Information . VGVFCs2mA/0.jpg' alt='Qiagen Plasmid Miniprep Kit Manuals Library' title='Qiagen Plasmid Miniprep Kit Manuals Library' />Water has a high dielectric constant, which makes it fairly difficult for the Na and PO3 to come together.Ethanol on the other hand has a much lower dielectric constant, making it much easier for Na to interact with the PO3, shield its charge and make the nucleic acid less hydrophilic, causing it to drop out of solution.The role of temperature in ethanol precipitationIncubation of the nucleic acidsaltethanol mixture at low temperatures e.C is commonly cited in protocols as necessary in protocols.However, according to Maniatis et al Molecular Cloning, A Laboratory Manual 2nd Edition 2nd edition I need to get a newer version, this is not required, as nucleic acids at concentrations as low as 2.L will precipitate at 0 4.C so incubation for 1.The wash step with 7.This step is to wash any residual salt away from the pelleted DNA.A few tips on ethanol precipitationChoice of salt.Use Sodium acetate 0.M final conc, p. H 5.DNA precipitations.Use Sodium chloride 0,2.M final conc for DNA samples containing SDS since Na.Cl keeps SDS soluble in 7.DNA. Use Lithium Chloride 0.M final conc for RNA.This is because 2.RNA precipitation and Li.Cl is more soluble in ethanol than Na.Ac so will not precipitate, but beware chloride ions will inhibit protein synthesis and DNA polymerase so Li.Cl is no good for RNA preps for in vitro translation or reverse transcription.In these cases, use Na.Ac. Use Ammonium acetate 2.M final conc for the removal of d.NTPs, but do not use for preparation of DNA for T4 polynucleotide kinase reactions as ammonium ions inhibit the enzyme.To increase the yield in precipitations of low concentration or small nucleic acid pieces less than 1.Add Mg. Cl. 2 to a final concentration of 0.MIncrease the time of incubation ice before centrifugation to 1 hour.This explanation should bring you up to speed on how the ethanol precipitation works.If you want to learn more about the ins and outs of ethanol precipitation and other DNA cleanup approaches, you might want to check out theseMore articles on Ethanol precipitation.
0 Comments
Canon Lide 50 Driver For Windows 7' title='Canon Lide 50 Driver For Windows 7' />Canon Scanner Drivers Download for Windows 1.XP, Vista. It is easy to find the right driver for your installed device.We have the most supported Canon Scanner Drivers available for Free Download.And for the most popular Canon products and devices.Use our custom search to find popular Canon Scanner models.You can also search our whole driver archive to find the exact driver that fits your needs.Browse the list below of the most popular Canon Scanner devices to find yours.Or go for automatic driver updates and get the correct Windows driver with just one click.The best way to fix your PC to run at peak performance is to update your drivers.Below is a list of our most popular Canon Scanner support software and device drivers.First select the Model Name or Number of your device.Canon Lide 50 Driver For Windows 7' title='Canon Lide 50 Driver For Windows 7' />Religion.Various formally approved collections of scriptures, including Biblical canon, among various Jewish and Christian communities Chinese Buddhist canon, used.Canon Inc., Kyanon Kabushikigaisha is a Japanese multinational corporation specialized in the manufacture of imaging and optical.Sometimes people find workarounds for problems like this, that there is no driver support for CanoScan LIDE 30 for Windows 7 64bit.So, Im asking here if anyone has.Next click the Download button.If you do not find the right match, type the model in the search box below to Search our Canon Device Driver Support Database.You may see different versions.Choose the best match for your PC.Need more help Discuss driver problems and ask for help from our community on our Free Driver Help Forum.Or you can request a driver and we will find it for you.We employ a team from around the world.They add hundreds of new drivers to our site every day.Canon Cano. Scan Li.DE 2. 20 Compact Scanner Amazon.Computers Accessories.Superior quality scanning.Effortlessly take care of all your home photo and document scanning needs with this fast, superior quality scanner.It offers high resolution scanning up to 4. Repair My Backup 4 20 Keygen Crack . CIS sensor capturing crisp text, clear photos with an exceptional level of detail and accurate colours thanks to the scanners 4.Scanning is quick too, with an A4 3. Video Center. Welcome to the Asphalt Kingdom Video Center. Choose your category below More About Our Videos. The Asphalt Kingdom video center provides a variety of videos, available in 4 categories. Our sales tips videos helps you improve your salesmanship with an in depth tutorial on what to do when youre going door to door to sell your sealcoating services. We also provide videos on the general offerings of Asphalt Kingdom and how we can help you, whether youre a homeowner, contractor or property owner. Our crack repair videos shows you how easy it is to fill asphalt cracks with our equipment. Our sealcoating videos and tutorials show you how easy sealcoating is, as well as how to do several key tasks such as emptying the tank, using the transfer kit, and how to agitate your sealer. The line striping videos show you the benefits and abilities of our parking lot striping equipment. Start a Business Videos. If youve wanted to start your own business then asphalt maintenance is definitely a lucrative business to get into. Many homeowners have asphalt driveways and many businesses have asphalt parking lots. The flexible nature of this material proves to be a great paving material, but as with most things, it needs maintenance. Our business solutions, such as the Business in a Box and our free marketing package, allow you to create your own business. We even help entrepreneurial teenagers and college students learn the ins and outs of the business. Here we offer you advice on how to improve your business, whether its by brushing up on your door to door salesmanship or by perfecting your technique with a sealcoating video. You can also find more tips by contractors on our blog. Sealcoating Equipment Product Demos. At AK, we pride ourselves on our easy to use, high quality equipment and products. These overviews of our products will give you a quick rundown of what our products will offer you and what benefits they have. Whether youre looking for a new piece of crack repair equipment, or want to see the quick specs on our sealer and crack filler, we have a video to help explain the details for you. Asphalt Tutorials. Our how to videos show you the proper technique for certain equipment operations, such as replacing the seal on a cast iron pump, or using the AK Transfer Kit. About Us. Crafco is the worlds leading manufacturer in quantity and diversity of packaged pavement preservation products for asphalt and concrete such as hot. We even have a video on how to assemble our popular AK1. If youre looking for even more videos, all of our videos can be found on the Asphalt Kingdom You. Tube channel. Subscribe to our channel for notifications on new videos and to have our latest videos on your You. Tube homepage. Free Expert Advice. Sealcoating videos are only one of the ways that we try to help homeowners, contractors, and property owners take care of their asphalt surfaces. We also provide free e mail courses and online guides. Want to talk to a real person Call us for free expert advice through our toll free number 1 8. If you need help with any of your projects, whether it is crack repair, pothole fixing, sealing, or line striping, we have the knowledge and know how to help you find the right products and tools you need to get professional looking results. Learn more about our successful history by reading customer testimonials or our case studies. We have over a decade of experience helping people take care of their asphalt, and we hope to continue that legacy with our videos, superior customer service, and professional grade products and equipment. Complete package that includes a machine simulation teaches the student the setup and operation of CNC machines. b CNC program simulation and gcode debugger. Practice the Past Progressive or Past Continuous and Past Simple Tenses, using this ESL Grammar Interactive Monkey Fun Game Activity for preintermediate was working. The present continuous tense is one that students will learn after learning the first three simple tenses that is simple present, past and future. When teaching. How to Teach Present Continuous Alternative Approach. The present continuous tense is one that students will learn after learning the first three simple tenses that is simple present, past and future. When teaching additional tenses it is important to highlight what time period is referred to and also in what situations students would used each tense. Warm up. Have students practice using the simple present tense as they volunteer to answer questions or play fruit basket to encourage students to make sentences of their own. When you have finished draw an image on the board illustrating the simple present tense as a reference point and ensure that students understand when to use the present tense. Introduce Present Continuous. Give students some example sentences using the present continuous tense. By using pairs of sentences in your examples, you can show when to use the present simple tense and when to use the present continuous tense. A good example might be I play baseball every day. and I am playing baseball with Ben right now. See if students understand the difference in meaning between the two and ask for translations. It is important to illustrate on the board the difference between this tense and other tenses your students have learned. The best way to do this is by drawing a simple chart. On the board under your image illustrating the present tense, draw a similar image to illustrate the present continuous tense. You can also discuss the fact that the present tense is used when talking about daily routines while the present continuous tense is used when talking about specific non routine actions and future plans. Practice Present Continuous. Give students some basic practice exercises so they can get used to changing present simple verbs into the present continuous tense. This can be done in the form of a fill in the blank worksheet but this would also be an appropriate topic to use battleship for speaking practice. Students can use the battleship grid to change I, You, play soccer, speak English, etc. I am playing soccer. and You are speaking English. If students have played this game before, it might take approximately thirty minutes to explain and play twice. Practice More. Phone conversations would be a real life situation where the present continuous tense is used quite often so in order to give students practice with this tense as well as basic phone conversation, make a short model dialogue for students to practice in pairs. This could be very simple where Student A asks Student B for someone and Student B replies Im sorry. Hes not here right now. Hes playing baseball with Ben. It could also be more complex. Adjust the conversation to suit your students ability level and encourage them to create part of the conversation, preferably the present continuous part of the conversation, on their own. Have students volunteer to present their dialogues to the class at the end of the activity. Produce. Ask students to imagine a situation and then write three to five present continuous tense sentences to describe it without saying the location. Students can then work in pairs or groups to guess the locations of different scenarios. An example may be I am sitting. I am listening to my music. I am looking out the window. I am moving. It is very simple but students might then guess the location as in a car, on a bus, in a train, or on a plane. Review Present Continuous Any activity you have done for this topic can be used as a review activity at the end of the lesson. You may also want to get students moving if they have been sitting down for the entire class period, especially after a writing exercise, so you can have students form a circle. Students should take turns making present continuous sentences and then calling on students to make the next sentence. You can continue this activity until everyone has made a sentence or until the bell rings. If you want to ensure that everyone makes at least one sentence, you can have students start sitting down after their turns. This is not recommended for large classes because then lots of students will be sitting down with nothing to do towards the end of the activity. Present continuous is a more complex tense that students may initially have difficulty understanding when to use but the more practice they have using it, the better off they will be. Telugu Songs Download Telugu movie songs online. Browse Telugu movies MP3 songs, Telugu music albums songs free. Listen to latest or old Telugu movie song and. Wap Toplist wap toplist Wap ranking Wapmaster site mobile wap toplist Free traffic wap traffic wap portal add your site world 1 toplist best toplist adult toplist free toplist Hotwapi. Hairy Maclary And Zachary Quack Attack ArcadeK8 CLASSROOM LIBRARY COLLECTIONS 20172018 Catalog. NEW Expanded Aligned Collection Selection. NO MINIMUM ORDER 18009226066 www. akjeducation. com. 9781869192457 1869192451 Klip En Klei, Marthinus Versfeld 9781606641439 1606641433 Robin, Frances Hodgson Burnett, Hamilton Williamson 9781436797948 1436797942 Cartas. K 8 Classroom Library Collections 2. AKJ Education. K 8 Classroom Library Collections 2. Published on Jan 3. Expanded collection selection, collections aligned to popular classroom curriculums, new author partnerships, and more All at 3. 0 off retai. Proceedings fo the 7th European Conference on Games Based Learning ECGBL 2. ACPILProceedings fo the 7th European Conference on Games Based Learning ECGBL 2. Published on Sep 1. Three and a half minutes into his talk, Pinker presents a chart based on Lawrence Keeleys War Before Civilization The Myth of the Peaceful Savage. Dissidia Final Fantasy NT is a fastpaced threeonthree arcade combat game featuring characters from Square Enixs beloved roleplaying franchise, coming to the. Films. info button v 1. Vote for ice. Films. IE 6 lt link relstylesheet typetextcss mediascreen hrefhttp globolister. Download Anime Dragon Ball Sub Indonesia MovieDragon Ball Z Dead Zone. Dragon Ball Z Dead Zone, originally released theatrically in Japan as simply Dragon Ball Z and later as Dragon Ball Z Return My GohanJapanese Z ,Hepburn Doragon Bru Zetto Ora no Gohan o Kaese for its Japanese VHS and Laserdisc release, is the fourth anime film in the Dragon Ball franchise and the first one under the Dragon Ball Z moniker. It was originally released in Japan on July 1. Toei Manga Matsuri film festival along with the 1. Himitsu no Akko chan, the first Akuma kun movie, and the film version of Kidou Keiji Jiban. Despite some continuity inconsistencies, Dead Zone acts as a prelude to the series, and is the only film to get a complete follow up during Dragon Ball Z, with the Garlic Jr. The film opens with Piccolo being attacked and supposedly killed by a group of shadowed fighters. Chi Chi along with her father, Ox King, and Gohan are attacked by the same group. Goku is out fishing during the attack and after sensing danger, he returns only to find his son has been kidnapped. Search the worlds information, including webpages, images, videos and more. Google has many special features to help you find exactly what youre looking for. Episode Pulau Manusia Ikan. Download Film Anime One Piece Episode 523 Bahasa Indonesia Download Film Anime One Piece Episode 524 Bahasa Indonesia. Gintama Episode 332 Subtitle Indonesia Gintama Pororihen Episode 4 Subtitle Indonesia Download. Download Anime Subtitle Indonesia. Fear The Walking Dead Season 3 episode 15 16 tamat subtitle indonesia. The group responsible for the attack are Garlic Jr. s henchmen, and it is later revealed that their reason for kidnapping Gohan was to retrieve the four star Dragon Ball that was attached to his hat. After gathering the remaining Dragon Balls, Garlic Jr. Shenron and he immediately wishes for immortality. Shenron, never having had to grant this type of demand before, grants Garlic Jr. A furious Goku arrives to try to take back his son, but soon discovers Garlic Jr. s new power. Kami makes an appearance which surprises Garlic Jr., who thought that his henchmen had killed Piccolo and meets up with Goku, and describes a brief history of Garlic Jr. Garlic. Goku then proceeds to try to find Gohan when he is attacked by the villains gang, whilst Kami faces Garlic Jr. After briefly fighting, Krillin and Piccolo arrive to help. Piccolo gets revenge for being attacked earlier by defeating Garlic Jr. s henchman Sansho, while Goku manages to defeat the other two henchmen, Ginger and Nicky. Meanwhile, Kami is getting brutally outmatched by Garlic Jr., until Goku and Piccolo rescue him. With Garlic Jr. s newly obtained immortality and a new muscular second form, Goku Piccolo are forced to work together and are able to outclass him. Goku and Piccolo then prepare to fight each other, but then Garlic Jr., angry at his defeat, unleashes his ultimate attack, opening up a portal into another dimension a void of darkness known as the Dead Zone, created by Garlic Jr. s father, Garlic and the same place Kami banished Garlic in, intending to suck his enemies into the void. Before he could claim victory, Gokus son, Gohan, becomes enraged witnessing his father and friends in danger and releases his latent energy, blasting Garlic Jr. Remarkably, in the end of it all, Gohan does not remember a thing of what happened, instead believing that his father defeated Garlic Jr., and with amazement, Goku figures his son is no ordinary boy, but one with a great hidden potential. Download Anime Dragon Ball Sub Indonesia EmpressAt the end of the film, Piccolo is seen from above, looking down on Goku and his friends and vows that one day, he will defeat Goku. A fourth English dub released in Malaysia by Speedy Video features an unknown cast. Furukawas voice from the original Japanese version is retained in the AB Groupe dub at the beginning of this film, when Piccolo screams and destroys a large rock formation. In the Funimation English dubs credits, Christopher R. Sabat is miscredited as playing the Ox King, a mistake carried over into the Funimation dub of The Worlds Strongest. English dub soundtrackseditThe 1. Pioneer release kept the original Japanese music. Funimations 2. 00. Mark Menza. The remastered releases contain both English audio tracks with the U. S. soundtrack and original Japanese score. English releaseseditThe film was licensed in North America by Funimation Entertainment and the home video rights were sub licensed to Pioneer Home Entertainment. Pioneers dub used the same voice cast as the TV series did at the time, and was dubbed by Ocean Productions. They released the film as Dead Zone, and later retitled it Dead Zone Vortex for its television airings. As a bonus feature on the Pioneer DVD, deleted scenes from the original episodes 1 and 9 are shown in Japanese with English subtitles, as these two episodes were yet to be dubbed in full at the time. In North America, Pioneers dub was released on VHS, Laser. Disc and DVD on December 1. Once their sub license expired, Funimation re released the film on DVD on May 3. Funimations voice cast. 3 Funimation also released the movie on November 1. First Strike, also containing The Worlds Strongest and The Tree of Might. It was later remastered and released in a Double Feature set with The Worlds Strongest on Blu ray and DVD on May 2. The film was released to DVD again on November 1, 2. Dragon Ball Z movies. 4AB Groupe, a French company that holds the license to the Dragon Ball franchise in most of Europe, licensed and dubbed the movie, which they re titled In Pursuit of Garlic. 5 This dub featured a voice cast that was unknown for years, but it is now believed that English speaking voice actors in France were involved with this dub. 1In Pursuit of Garlic aired on TV in the Netherlands, the United Kingdom, and Ireland, and was sold on DVD in the Netherlands by Bridge Entertainment Group. Speedy Video, a Malaysian based company, released the film on Video CD, here subtitled The Vengeance of the Demon King. Speedy also released the Pioneer English adaptation on VCD. Both the AB Groupe and Speedy dubs are notoriously known for inaccurate translations e. Piccolo was called Big Green in the AB Groupe dub and dialogue that did not fit the mouth flaps. Referencesedit abDragon Ball Z Big Green Dub Cast Behind The Voice Actors. Retrieved 2. 01. 6 0. Nishio, Daisuke 1. Dragon Ball Z The Movie Dead Zone, Geneon Pioneer, retrieved 2. Nishio, Daisuke 2. Dragon Ball Z The Movie Dead Zone, Funimation Prod, retrieved 2. Dragon Ball Z Movie Pack Collection One, Funimation Prod, 2. Kanzenshuu View topic Where to buy the Big Green Dub DVDs from. Retrieved 2. 01. 6 0. External linksedit. Call for Papers International Journal of Advanced Research in Computer Engineering Technology. Dear AuthorResearcher,International Journal of Advanced Research in Computer Engineering Technology IJARCET invites you to submit your research paper for publishing in Volume 6, Issue 1. November 2. 01. 7. Scope Topics. International Journal of Advanced Research in Computer Engineering Technology IJARCET is a monthly open access journal that publishes articles which contribute new results in all areas of the Computer Science Computer Engineering. The journal is devoted to the publication of high quality papers on theoretical and practical aspects of computer science and computer Engineering. The goal of this journal is to bring together researchers and practitioners from academia and industry to focus on Computer science Computer Engineering advancements, and establishing new collaborations in these areas. Original research papers, state of the art reviews are invited for publication in all areas of Computer Science Computer Engineering. Online Publication Fee Detail For Foreign Author 5. USD for publishing. For Indian author Rs. Paper Submission Authors are invited to submit papers through E mail at ijarcetgmail. Submissions must be original and should not have been published previously or be under consideration for publication while being evaluated for this journal. Paper must be in IJARCET Templete Topics considered include but are not limited to Accessible Computing Algorithms and Computation Theory Artificial Intelligence and Soft Computing Bioinformatics and Biosciences Computer Architecture Computer Graphics and Animation Computer Science Information Technology Education Cryptography and Information security Data Communication and Computer Networks Data Mining and Knowledge Management Process Database Management Systems Design Automation Digital Signal and Image Processing Electronic Commerce Embedded Systems Genetic and Evolutionary Computation Health Informatics High Performance Computing Information Retrieval Internet Engineering Web services Management Information Systems Measurement and Evaluation Microarchitecture Multimedia and Applications Operating Systems Programming Languages Security, Privacy and Trust Management Simulation and Modeling Software Engineering Ubiquitous computing Wireless and Mobile networks. Important Dates. Submission deadline 3. October, 2. 01. 7Notification 5th November, 2. Final manuscript due 8th November, 2. Publication date 1. November, 2. 01. 7With Warm Regards,Editor in chief. Email ijarcetgmail. Radiation Efficiency Of Patch Antenna Bandwidth DetectsRadiation Efficiency Of Patch Antenna Bandwidth CalculationPulse. Larsen Antennas Catalog 2. Pulse Electronics. Pulse. Larsen Antennas Catalog 2. Published on Mar 1. Pulse. Larsen Antennas is pleased to bring you the new, improved Antenna Source. Book ASB, Volume 1. The goal of the ASB is to provide you. IEEE Antennas and Propagation Magazine actively solicits feature articles that describe engineering activities taking place in industry, government, and universities. MICROWAVE WEEK INVITATION FROM THE GENERAL CHAIR. Bienvenue Montral Yes indeed, we are all very much excited about IMS2012, which is finally coming to Montral. Directivity is explained, which is a measure of the directionality of an antennas radiation pattern. Particular emphasis is paid to peak. In the short term, exposure to fallout will cause you to become ill, suffering acute radiation syndrome. Youll suffer rapid cellular degradation and DNA damage and. Download Update. Star Update. Star. Download the. free trial version below to get started. Double click the downloaded file. Update. Star is compatible with Windows platforms. MUST READ To help you prepare for US Visa Interview, here are the sample F1 Visa Interview Questions and Answers. If your dream is to study in the US, all starts. Issuu is a digital publishing platform that makes it simple to publish magazines, catalogs, newspapers, books, and more online. Easily share your publications and get. Update. Star has been tested to meet all of the technical requirements to be compatible with. Windows 1. 0, 8. 1, Windows 8, Windows 7, Windows Vista, Windows Server 2. Windows. XP, 3. 2 bit and 6. Simply double click the downloaded file to install it. Even more Account Options. Sign in Search settings. Update. Star Free and Update. Star Premium come with the same installer. Update. Star includes support for many languages such as English, German, French, Italian, Hungarian, Russian and many more. You can choose your language settings from within the program. Extremely Useful F1 Visa Interview Questions and Answers Visa interview is a face to face interaction between two individuals. No matter the level of financial security and educational background backs up for you, preparation is a must. Know the program and how it fits you The more you know about your program, the more focused your answers will be. You should be able to prove yourself that you have enough skills, background, and ability to advance your studies or work in the United States. Successful visa interviews are not just based on documentation and background. Personality, enthusiasm, confidence, positive outlook, appearance, and clear communication count heavily in a visa interview. Here is a list of possible F1 visa interview questions. We have also provided some tips and sample answers to help you construct your own unique answers. Q What do you know about American universities The officer wants to know what made you do decide to study in American universities. You should highlight few features of US universities. Sample Answers According to my research, American Universities focus primarily on practice of the taught knowledge at their institutes and provide students with numerous research opportunities. Speaking from experience of alumni, programs implemented in American Universities are very well designed and renowned for their impeccable quality and commendable structure that focuses on building skills. American universities are well known for their quality of education and research opportunities. Their programs are well designed with equal importance on theoretical knowledge along with strong practical skills. Q Why did you choose to study in US Why study in US, why not UK or Canada The visa officer is looking for a justifiable reason as to what convinced you to pursue higher studies in US. You will have to tell what features of the American education system appeal to you compared to other countries. Sample Answers Academic experience from a US university is highly valued around the world. Also, US universities offer excellent research opportunities through many assistant ship programs. On top of that, having global exposure tremendously helps to advance my career. The American education system has been deemed the best amongst all others and I believe their programs are well structured and suitable for the field I would like to step in. Universities in U. S. hail a fine blend of theoretical and practical knowledge. To develop my practical skills, enrolling in a U. S. university is the best option. Q What will be your specialization in MS What will be your major in MS Answer appropriately eg I will be majoring in Computer ScienceQ Why do you want to do Masters State why you want to do Masters in a particular field with enthusiasm and purpose. Your answer should reflect confidence in your decision and should outline why doing Masters in your field is necessary for you. Sample Answers Pursuing a Masters degree in Computer Science will allow me to gain more technical knowledge and help me find more opportunities and better prospects. In this competitive world, higher qualification in a field will serve greatly to give me a better chance to join the industry of my choice as a long term career prospect. Q What are your plans after graduation Your answer should show concrete evidence that you do not plan to live in the U. S. after studies but to return to your home country. Sample Answers I plan to return to my country and begin working in a firm in my city as a software developer as the demand for individuals in this profession keeps increasing. After completing my course in computer science, I will return to join the ever growing IT industry in my country. We have great opportunities here in the field of my study. The advance degree I will be pursuing from US will be a great asset in advancing my career here in my country. Q Do you have your GRETOEFLIELTSGMAT score reports Answer appropriately Provide originalsQ Why are your GRETOEFLIELTS scores low Truthfully explain what mistakes you made and where you stumbled. Do not make excuses or cover up your errors. Instead, own up to it and explain a proper and genuine reason as to why you were unable to perform that well. Sample Answers The test took place during my college examinations which is why I was not thoroughly prepared. Despite my low GRE score, my verbal TOEFL score is pretty good. I answered the first few questions wrong which caused my score to fall. Despite performing well in the latter part of the test, I was not able to substantially improve it. Q What was your major in undergrad What was your specialization in Bachelors Answer appropriately eg I did Bachelors in Electrical EngineeringQ Why change your academic background now You must know what made you change your area of specialization. It is necessary to have a genuine reason as to what convinced you to switch your major. Sample Answers I have always been more passionate about environmental science and pursuing this degree will allow me to step into a field which I truly enjoy instead of continuing one I chose because of high pay. There is much more demand and need of this profession in my country compared to the one I am already in. Q Why do you have several backlogs You should convince the officer that you can study in the US despite these shortcomings. Convince him that you can do better and state confidently how so. Sample Answers During a certain period, I was unable to manage my studies and co curricular activities which is what damaged my performance report. But I have made promises and have determination to not repeat my mistakes. I have made amends to ensure I can continue studies in the US by selecting subjects I am extremely proficient and well versed in. I am sure I can handle the pressure now. Q Have you been to the States beforeHave you ever been outside India Answer appropriately eg No I have never been outside IndiaQ Can I see your Bachelors diploma Answer appropriately. Q When and from which university did you graduate Answer appropriately eg I graduated in XXXXyear from XYZ universityQ Can I see your marksheetstranscripts Answer appropriately. Q What all universities did you apply Answer appropriately eg I applied to 3 universitiesQ Which universities did you get admissionAnswer appropriately eg I got admission from all 3 universitiesQ Which university are going to Answer appropriately. Q Why this university The officer wants to know what your reason for choosing that specific university is. You should Provide a solid and justifiable reason for pursuing your studies at this university. Sample Answers I have interacted with few current students who are pursuing their Masters at this University. They advised its one of the Universities that offers great research opportunities in the field I will be studying and on top of that, it has outstanding faculty. I have been accepted to a few other universities as well but this one appealed the most to me. It is because the courses that are offered at this university perfectly match my area of interest that is Data Analytics. Other institutes did not offer this field as a separate course, which is why this university became my top priority. Q Did you get any scholarship Answer accordingly eg YesI was awarded X dollars scholarshipQ Did you contact any professors from that university Answer appropriately eg Yes I have been in touch with couple professorsQ Do you happen to remember those professors namesAnswer appropriately eg Name few professorsQ Do you happen to know about the city you will be going Answer appropriately e. I heard Houston weather is very much similar to Indian weather. So that definitely helps me to get adjusted to it pretty quickly. Q Do you have family in the US Answer appropriately. Q Do you have friends in USAnswer appropriately. Q What is your fatherAnswer appropriately. Q Do you have siblings Answer appropriately. Q Who is sponsoring your studies Answer appropriately eg My father is sponsoring my educationQ What is your fathers income Answer appropriately. Q Can I see tax returns Answer appropriately. Q Can I see Bank StatementsPassbookAnswer appropriately. Q Did you take any loans Answer appropriately If yes, give the officer your loan approval letterQ Where do your parents liveAnswer appropriately. Q Why do you think we should give you visa to study in the US |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |